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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: BRAF-Mutated Melanoma Cell Lines Develop Distinct Molecular Signatures After Prolonged Exposure to AZ628 or Dabrafenib: Potential Benefits of the Antiretroviral Treatments Cabotegravir or Doravirine on BRAF-Inhibitor-Resistant Cells
doi: 10.3390/ijms252211939
Figure Lengend Snippet: Characterization of phenotypes in melanoma cell populations resistant to dabrafenib or AZ628. This figure illustrates various features of melanoma cell populations with respect to signaling pathways, cell cycle regulation, and susceptibility to cell death. Receptor tyrosine kinase (RTK): including tyrosine-protein kinase receptor UFO (AXL) and mesenchymal–epithelial transition factor (c-Met). RAS/RAF/MAPK signaling pathway: detailing expression levels of V-raf murine sarcoma viral oncogenes (RAF) including BRAF and CRAF isoforms, as well as total extracellular signal-regulated kinases (ERK) expression. Pro-proliferative proteins: including phosphorylation levels of retinoblastoma protein (pRb) and M-phase inducer phosphatase 3 (pCDC25C), along with cyclin D1 and cyclin A2 expression. Apoptosis markers: displaying levels of cleaved poly (ADP-ribose) polymerase (cPARP) and mitochondrial carrier homolog 2 (MTCH2). Ferroptosis markers: highlighting the expression of transferrin and heme oxygenase-1 (HO-1), both of which contribute to increased intracellular iron. Levels of ferritin heavy chain 1 (FTH1), glutathione peroxidase 4 (GPX4), and cystine-glutamate antiporter (SLC7A11) are also presented.
Article Snippet: Following blocking, the membranes were incubated overnight at 4 °C on a shaker in a 5% BSA solution containing the following primary antibodies: Transferrin (A1448), Ferritin heavy chain (A19544), cleaved PARP-P25 (A19612), CDKN1B/p27KIP1 (A19095), CDKN2A/p16INK4a (A11651), Cyclin A2 (A19036), Bcl-xL (A0209), Heme Oxygenase 1 (A19062), ERK 1/2 (A16686), pERK 1/2 (AP0974), SLC7A11/xCT (A2413), C-Raf (A19638) from ABclonal (Woburn, MA, USA); Cyclin D1 (GTX634347),
Techniques: Expressing